Atividade anti-protozoário in vitro do p-Cimeno sobre Leishmania amazonensis

Jesieli Beraldo-Borrazzo, Adriana Oliveira dos Santos, Benício Alves de Abreu Filho, Lucindo José Quintans-Júnior, Celso Vataru Nakamura

Resumo


As doenças tropicais se constituem em um grave problema de saúde pública, por serem negligenciadas e atingirem especialmente as populações mais pobres. Entre os principais problemas mundiais de saúde, a leishmaniose se destaca por apresentar altas taxas de mortalidade. Grandes esforços tem sido empregados na busca por novos medicamentos nas ultimas décadas, sendo o uso da fitoterapia investigado com grande interesse, devido às plantas serem consideradas uma ampla fonte de compostos com potencial biomedicinal. A atividade anti-protozoário da substância p-Cimeno sobre formas promastigotas e amastigotas axênicas de Leishmania amazonensis, foi avaliado. Após 72 horas de cultivo, o p-Cimeno apresentou concentração inibitória de 50% (CI50) igual à 65,0 µg/mL e 55,0 µg/mL sobre as formas promastigotas e amastigotas axênicas,  respectivamente. Análises de citotoxicidade do p-Cimeno, foram realizadas por meio do método colorimétrico da sulforodamina-B sobre macrófagos J774G8 e a concentração citotóxica para 50% das células (CC50) foi de 54,0 µg/mL. Os dados obtidos indicam efeito antiparasitário do p-Cimeno sobre Leishmania amazonensis, principalmente sobre sua forma amastigota, sendo considerado potencial candidato para novos estudos contra o parasito.

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